peripheral nerves Search Results


buzsaki32 h32 21  (NeuroNexus Technologies)
94
NeuroNexus Technologies buzsaki32 h32 21
Buzsaki32 H32 21, supplied by NeuroNexus Technologies, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peripheral+nerves/pm32508613-165-24-23?v=NeuroNexus+Technologies
Average 94 stars, based on 1 article reviews
buzsaki32 h32 21 - by Bioz Stars, 2026-07
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nav1 8  (StressMarq)
90
StressMarq nav1 8
Nav1 8, supplied by StressMarq, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peripheral+nerves/10__9734_slash_jabb_slash_2014_slash_11452-69-36-22?v=StressMarq
Average 90 stars, based on 1 article reviews
nav1 8 - by Bioz Stars, 2026-07
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human peripheral nerve pericyte  (ScienCell)
90
ScienCell human peripheral nerve pericyte
(A) Immunostaining of human <t>peripheral</t> nerve microvascular endothelial cells (PnMECs) for snRNPs. Nuclear snRNP expression in monolayer PnMECs decreased after incubation with GBS-IgG with snRNP antibodies or anti-snRNP monoclonal antibody compared with after cont-IgG or cont-Ab. GBS-IgG: IgG purified from serum samples of 20 patients with U1-snRNP autoantibody–positive GBS; cont-IgG: IgG purified from serum samples of 10 healthy controls; RNP antibody: commercial snRNP monoclonal antibody; cont-Ab: commercial anti-rabbit IgG. Scale bar, 50 μm. (B) Incubation of GBS-IgG with snRNP antibodies or anti-snRNP antibody caused a significant increase in 10-kDa dextran permeability, compared with that with cont-IgG or cont-Ab, using a monolayer coculture model consisting of FH-BNB cells and <t>pericytes.</t> (C) GBS-IgG with anti-snRNP antibody induced a significant increase in the permeability of FH-BNB compared with cont-IgG, GBS-IgG without anti-snRNP antibody, or GBS-IgG after the reduction of U1-snRNP antibody from GBS-IgG with U1-snRNP antibody. (D) Incubation with GBS-IgG or snRNP antibodies leads to a decrease in claudin-5 and an increase in 10-kDa dextran permeability compared with cont-IgG using the spheroid model, which consists of both FH-BNB cells and pericytes (Left). Scale bar, 50 μm. Permeability of 20-kDa dextran increased after exposure to GBS-IgG or snRNP antibodies compared with cont-IgG using a microfluidic in vitro BNB coculture model (Right). Scale bar, 100 μm. BNB = blood-nerve barrier; U1-snRNP = U1-small nuclear ribonucleoprotein.
Human Peripheral Nerve Pericyte, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peripheral+nerves/pmc12092546-78-7-45?v=ScienCell
Average 90 stars, based on 1 article reviews
human peripheral nerve pericyte - by Bioz Stars, 2026-07
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sprint peripheral nerve stimulation (pns) system  (SPR Therapeutics LLC)
90
SPR Therapeutics LLC sprint peripheral nerve stimulation (pns) system
(A) Immunostaining of human <t>peripheral</t> nerve microvascular endothelial cells (PnMECs) for snRNPs. Nuclear snRNP expression in monolayer PnMECs decreased after incubation with GBS-IgG with snRNP antibodies or anti-snRNP monoclonal antibody compared with after cont-IgG or cont-Ab. GBS-IgG: IgG purified from serum samples of 20 patients with U1-snRNP autoantibody–positive GBS; cont-IgG: IgG purified from serum samples of 10 healthy controls; RNP antibody: commercial snRNP monoclonal antibody; cont-Ab: commercial anti-rabbit IgG. Scale bar, 50 μm. (B) Incubation of GBS-IgG with snRNP antibodies or anti-snRNP antibody caused a significant increase in 10-kDa dextran permeability, compared with that with cont-IgG or cont-Ab, using a monolayer coculture model consisting of FH-BNB cells and <t>pericytes.</t> (C) GBS-IgG with anti-snRNP antibody induced a significant increase in the permeability of FH-BNB compared with cont-IgG, GBS-IgG without anti-snRNP antibody, or GBS-IgG after the reduction of U1-snRNP antibody from GBS-IgG with U1-snRNP antibody. (D) Incubation with GBS-IgG or snRNP antibodies leads to a decrease in claudin-5 and an increase in 10-kDa dextran permeability compared with cont-IgG using the spheroid model, which consists of both FH-BNB cells and pericytes (Left). Scale bar, 50 μm. Permeability of 20-kDa dextran increased after exposure to GBS-IgG or snRNP antibodies compared with cont-IgG using a microfluidic in vitro BNB coculture model (Right). Scale bar, 100 μm. BNB = blood-nerve barrier; U1-snRNP = U1-small nuclear ribonucleoprotein.
Sprint Peripheral Nerve Stimulation (Pns) System, supplied by SPR Therapeutics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peripheral+nerves/pm29057550-49-11-17?v=SPR+Therapeutics+LLC
Average 90 stars, based on 1 article reviews
sprint peripheral nerve stimulation (pns) system - by Bioz Stars, 2026-07
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peripheral nerve stimulation therapy  (SPR Therapeutics LLC)
90
SPR Therapeutics LLC peripheral nerve stimulation therapy
(A) Immunostaining of human <t>peripheral</t> nerve microvascular endothelial cells (PnMECs) for snRNPs. Nuclear snRNP expression in monolayer PnMECs decreased after incubation with GBS-IgG with snRNP antibodies or anti-snRNP monoclonal antibody compared with after cont-IgG or cont-Ab. GBS-IgG: IgG purified from serum samples of 20 patients with U1-snRNP autoantibody–positive GBS; cont-IgG: IgG purified from serum samples of 10 healthy controls; RNP antibody: commercial snRNP monoclonal antibody; cont-Ab: commercial anti-rabbit IgG. Scale bar, 50 μm. (B) Incubation of GBS-IgG with snRNP antibodies or anti-snRNP antibody caused a significant increase in 10-kDa dextran permeability, compared with that with cont-IgG or cont-Ab, using a monolayer coculture model consisting of FH-BNB cells and <t>pericytes.</t> (C) GBS-IgG with anti-snRNP antibody induced a significant increase in the permeability of FH-BNB compared with cont-IgG, GBS-IgG without anti-snRNP antibody, or GBS-IgG after the reduction of U1-snRNP antibody from GBS-IgG with U1-snRNP antibody. (D) Incubation with GBS-IgG or snRNP antibodies leads to a decrease in claudin-5 and an increase in 10-kDa dextran permeability compared with cont-IgG using the spheroid model, which consists of both FH-BNB cells and pericytes (Left). Scale bar, 50 μm. Permeability of 20-kDa dextran increased after exposure to GBS-IgG or snRNP antibodies compared with cont-IgG using a microfluidic in vitro BNB coculture model (Right). Scale bar, 100 μm. BNB = blood-nerve barrier; U1-snRNP = U1-small nuclear ribonucleoprotein.
Peripheral Nerve Stimulation Therapy, supplied by SPR Therapeutics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peripheral+nerves/pmc05368033-256-9-3?v=SPR+Therapeutics+LLC
Average 90 stars, based on 1 article reviews
peripheral nerve stimulation therapy - by Bioz Stars, 2026-07
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peripheral nerve stimulator easymed instruments co  (EasyMed Services Inc)
90
EasyMed Services Inc peripheral nerve stimulator easymed instruments co
(A) Immunostaining of human <t>peripheral</t> nerve microvascular endothelial cells (PnMECs) for snRNPs. Nuclear snRNP expression in monolayer PnMECs decreased after incubation with GBS-IgG with snRNP antibodies or anti-snRNP monoclonal antibody compared with after cont-IgG or cont-Ab. GBS-IgG: IgG purified from serum samples of 20 patients with U1-snRNP autoantibody–positive GBS; cont-IgG: IgG purified from serum samples of 10 healthy controls; RNP antibody: commercial snRNP monoclonal antibody; cont-Ab: commercial anti-rabbit IgG. Scale bar, 50 μm. (B) Incubation of GBS-IgG with snRNP antibodies or anti-snRNP antibody caused a significant increase in 10-kDa dextran permeability, compared with that with cont-IgG or cont-Ab, using a monolayer coculture model consisting of FH-BNB cells and <t>pericytes.</t> (C) GBS-IgG with anti-snRNP antibody induced a significant increase in the permeability of FH-BNB compared with cont-IgG, GBS-IgG without anti-snRNP antibody, or GBS-IgG after the reduction of U1-snRNP antibody from GBS-IgG with U1-snRNP antibody. (D) Incubation with GBS-IgG or snRNP antibodies leads to a decrease in claudin-5 and an increase in 10-kDa dextran permeability compared with cont-IgG using the spheroid model, which consists of both FH-BNB cells and pericytes (Left). Scale bar, 50 μm. Permeability of 20-kDa dextran increased after exposure to GBS-IgG or snRNP antibodies compared with cont-IgG using a microfluidic in vitro BNB coculture model (Right). Scale bar, 100 μm. BNB = blood-nerve barrier; U1-snRNP = U1-small nuclear ribonucleoprotein.
Peripheral Nerve Stimulator Easymed Instruments Co, supplied by EasyMed Services Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peripheral+nerves/pmc06499052-97-0-3?v=EasyMed+Services+Inc
Average 90 stars, based on 1 article reviews
peripheral nerve stimulator easymed instruments co - by Bioz Stars, 2026-07
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tof-guard peripheral nerve stimulator acceleromyograph  (Organon Teknika Corporation LLC)
90
Organon Teknika Corporation LLC tof-guard peripheral nerve stimulator acceleromyograph
(A) Immunostaining of human <t>peripheral</t> nerve microvascular endothelial cells (PnMECs) for snRNPs. Nuclear snRNP expression in monolayer PnMECs decreased after incubation with GBS-IgG with snRNP antibodies or anti-snRNP monoclonal antibody compared with after cont-IgG or cont-Ab. GBS-IgG: IgG purified from serum samples of 20 patients with U1-snRNP autoantibody–positive GBS; cont-IgG: IgG purified from serum samples of 10 healthy controls; RNP antibody: commercial snRNP monoclonal antibody; cont-Ab: commercial anti-rabbit IgG. Scale bar, 50 μm. (B) Incubation of GBS-IgG with snRNP antibodies or anti-snRNP antibody caused a significant increase in 10-kDa dextran permeability, compared with that with cont-IgG or cont-Ab, using a monolayer coculture model consisting of FH-BNB cells and <t>pericytes.</t> (C) GBS-IgG with anti-snRNP antibody induced a significant increase in the permeability of FH-BNB compared with cont-IgG, GBS-IgG without anti-snRNP antibody, or GBS-IgG after the reduction of U1-snRNP antibody from GBS-IgG with U1-snRNP antibody. (D) Incubation with GBS-IgG or snRNP antibodies leads to a decrease in claudin-5 and an increase in 10-kDa dextran permeability compared with cont-IgG using the spheroid model, which consists of both FH-BNB cells and pericytes (Left). Scale bar, 50 μm. Permeability of 20-kDa dextran increased after exposure to GBS-IgG or snRNP antibodies compared with cont-IgG using a microfluidic in vitro BNB coculture model (Right). Scale bar, 100 μm. BNB = blood-nerve barrier; U1-snRNP = U1-small nuclear ribonucleoprotein.
Tof Guard Peripheral Nerve Stimulator Acceleromyograph, supplied by Organon Teknika Corporation LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peripheral+nerves/pm19440810-71-45-51?v=Organon+Teknika+Corporation+LLC
Average 90 stars, based on 1 article reviews
tof-guard peripheral nerve stimulator acceleromyograph - by Bioz Stars, 2026-07
90/100 stars
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peripheral erector spinae nerve block with ropivacaine naropin  (AstraZeneca ltd)
90
AstraZeneca ltd peripheral erector spinae nerve block with ropivacaine naropin
(A) Immunostaining of human <t>peripheral</t> nerve microvascular endothelial cells (PnMECs) for snRNPs. Nuclear snRNP expression in monolayer PnMECs decreased after incubation with GBS-IgG with snRNP antibodies or anti-snRNP monoclonal antibody compared with after cont-IgG or cont-Ab. GBS-IgG: IgG purified from serum samples of 20 patients with U1-snRNP autoantibody–positive GBS; cont-IgG: IgG purified from serum samples of 10 healthy controls; RNP antibody: commercial snRNP monoclonal antibody; cont-Ab: commercial anti-rabbit IgG. Scale bar, 50 μm. (B) Incubation of GBS-IgG with snRNP antibodies or anti-snRNP antibody caused a significant increase in 10-kDa dextran permeability, compared with that with cont-IgG or cont-Ab, using a monolayer coculture model consisting of FH-BNB cells and <t>pericytes.</t> (C) GBS-IgG with anti-snRNP antibody induced a significant increase in the permeability of FH-BNB compared with cont-IgG, GBS-IgG without anti-snRNP antibody, or GBS-IgG after the reduction of U1-snRNP antibody from GBS-IgG with U1-snRNP antibody. (D) Incubation with GBS-IgG or snRNP antibodies leads to a decrease in claudin-5 and an increase in 10-kDa dextran permeability compared with cont-IgG using the spheroid model, which consists of both FH-BNB cells and pericytes (Left). Scale bar, 50 μm. Permeability of 20-kDa dextran increased after exposure to GBS-IgG or snRNP antibodies compared with cont-IgG using a microfluidic in vitro BNB coculture model (Right). Scale bar, 100 μm. BNB = blood-nerve barrier; U1-snRNP = U1-small nuclear ribonucleoprotein.
Peripheral Erector Spinae Nerve Block With Ropivacaine Naropin, supplied by AstraZeneca ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peripheral+nerves/pmc09716230-69-12-19?v=AstraZeneca+ltd
Average 90 stars, based on 1 article reviews
peripheral erector spinae nerve block with ropivacaine naropin - by Bioz Stars, 2026-07
90/100 stars
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sensory feedback through peripheral nerve stimulation  (BioMimetic Therapeutics)
90
BioMimetic Therapeutics sensory feedback through peripheral nerve stimulation
(A) Immunostaining of human <t>peripheral</t> nerve microvascular endothelial cells (PnMECs) for snRNPs. Nuclear snRNP expression in monolayer PnMECs decreased after incubation with GBS-IgG with snRNP antibodies or anti-snRNP monoclonal antibody compared with after cont-IgG or cont-Ab. GBS-IgG: IgG purified from serum samples of 20 patients with U1-snRNP autoantibody–positive GBS; cont-IgG: IgG purified from serum samples of 10 healthy controls; RNP antibody: commercial snRNP monoclonal antibody; cont-Ab: commercial anti-rabbit IgG. Scale bar, 50 μm. (B) Incubation of GBS-IgG with snRNP antibodies or anti-snRNP antibody caused a significant increase in 10-kDa dextran permeability, compared with that with cont-IgG or cont-Ab, using a monolayer coculture model consisting of FH-BNB cells and <t>pericytes.</t> (C) GBS-IgG with anti-snRNP antibody induced a significant increase in the permeability of FH-BNB compared with cont-IgG, GBS-IgG without anti-snRNP antibody, or GBS-IgG after the reduction of U1-snRNP antibody from GBS-IgG with U1-snRNP antibody. (D) Incubation with GBS-IgG or snRNP antibodies leads to a decrease in claudin-5 and an increase in 10-kDa dextran permeability compared with cont-IgG using the spheroid model, which consists of both FH-BNB cells and pericytes (Left). Scale bar, 50 μm. Permeability of 20-kDa dextran increased after exposure to GBS-IgG or snRNP antibodies compared with cont-IgG using a microfluidic in vitro BNB coculture model (Right). Scale bar, 100 μm. BNB = blood-nerve barrier; U1-snRNP = U1-small nuclear ribonucleoprotein.
Sensory Feedback Through Peripheral Nerve Stimulation, supplied by BioMimetic Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peripheral+nerves/pm37820004-395-19-22?v=BioMimetic+Therapeutics
Average 90 stars, based on 1 article reviews
sensory feedback through peripheral nerve stimulation - by Bioz Stars, 2026-07
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finite-element model of a peripheral nerve and tripolar cuff electrode  (COMSOL Inc)
90
COMSOL Inc finite-element model of a peripheral nerve and tripolar cuff electrode
(A) Immunostaining of human <t>peripheral</t> nerve microvascular endothelial cells (PnMECs) for snRNPs. Nuclear snRNP expression in monolayer PnMECs decreased after incubation with GBS-IgG with snRNP antibodies or anti-snRNP monoclonal antibody compared with after cont-IgG or cont-Ab. GBS-IgG: IgG purified from serum samples of 20 patients with U1-snRNP autoantibody–positive GBS; cont-IgG: IgG purified from serum samples of 10 healthy controls; RNP antibody: commercial snRNP monoclonal antibody; cont-Ab: commercial anti-rabbit IgG. Scale bar, 50 μm. (B) Incubation of GBS-IgG with snRNP antibodies or anti-snRNP antibody caused a significant increase in 10-kDa dextran permeability, compared with that with cont-IgG or cont-Ab, using a monolayer coculture model consisting of FH-BNB cells and <t>pericytes.</t> (C) GBS-IgG with anti-snRNP antibody induced a significant increase in the permeability of FH-BNB compared with cont-IgG, GBS-IgG without anti-snRNP antibody, or GBS-IgG after the reduction of U1-snRNP antibody from GBS-IgG with U1-snRNP antibody. (D) Incubation with GBS-IgG or snRNP antibodies leads to a decrease in claudin-5 and an increase in 10-kDa dextran permeability compared with cont-IgG using the spheroid model, which consists of both FH-BNB cells and pericytes (Left). Scale bar, 50 μm. Permeability of 20-kDa dextran increased after exposure to GBS-IgG or snRNP antibodies compared with cont-IgG using a microfluidic in vitro BNB coculture model (Right). Scale bar, 100 μm. BNB = blood-nerve barrier; U1-snRNP = U1-small nuclear ribonucleoprotein.
Finite Element Model Of A Peripheral Nerve And Tripolar Cuff Electrode, supplied by COMSOL Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peripheral+nerves/10__1109_slash_tbme__2012__2194146-85-6-15?v=COMSOL+Inc
Average 90 stars, based on 1 article reviews
finite-element model of a peripheral nerve and tripolar cuff electrode - by Bioz Stars, 2026-07
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spinal cord-peripheral nerve co-culture system  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare spinal cord-peripheral nerve co-culture system
(A) Immunostaining of human <t>peripheral</t> nerve microvascular endothelial cells (PnMECs) for snRNPs. Nuclear snRNP expression in monolayer PnMECs decreased after incubation with GBS-IgG with snRNP antibodies or anti-snRNP monoclonal antibody compared with after cont-IgG or cont-Ab. GBS-IgG: IgG purified from serum samples of 20 patients with U1-snRNP autoantibody–positive GBS; cont-IgG: IgG purified from serum samples of 10 healthy controls; RNP antibody: commercial snRNP monoclonal antibody; cont-Ab: commercial anti-rabbit IgG. Scale bar, 50 μm. (B) Incubation of GBS-IgG with snRNP antibodies or anti-snRNP antibody caused a significant increase in 10-kDa dextran permeability, compared with that with cont-IgG or cont-Ab, using a monolayer coculture model consisting of FH-BNB cells and <t>pericytes.</t> (C) GBS-IgG with anti-snRNP antibody induced a significant increase in the permeability of FH-BNB compared with cont-IgG, GBS-IgG without anti-snRNP antibody, or GBS-IgG after the reduction of U1-snRNP antibody from GBS-IgG with U1-snRNP antibody. (D) Incubation with GBS-IgG or snRNP antibodies leads to a decrease in claudin-5 and an increase in 10-kDa dextran permeability compared with cont-IgG using the spheroid model, which consists of both FH-BNB cells and pericytes (Left). Scale bar, 50 μm. Permeability of 20-kDa dextran increased after exposure to GBS-IgG or snRNP antibodies compared with cont-IgG using a microfluidic in vitro BNB coculture model (Right). Scale bar, 100 μm. BNB = blood-nerve barrier; U1-snRNP = U1-small nuclear ribonucleoprotein.
Spinal Cord Peripheral Nerve Co Culture System, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peripheral+nerves/10__1111_slash_febs__12340-18702-33-20?v=Johns+Hopkins+HealthCare
Average 90 stars, based on 1 article reviews
spinal cord-peripheral nerve co-culture system - by Bioz Stars, 2026-07
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the peripheral nerve: structure, function and reconstruction  (Hampton Press)
90
Hampton Press the peripheral nerve: structure, function and reconstruction
(A) Immunostaining of human <t>peripheral</t> nerve microvascular endothelial cells (PnMECs) for snRNPs. Nuclear snRNP expression in monolayer PnMECs decreased after incubation with GBS-IgG with snRNP antibodies or anti-snRNP monoclonal antibody compared with after cont-IgG or cont-Ab. GBS-IgG: IgG purified from serum samples of 20 patients with U1-snRNP autoantibody–positive GBS; cont-IgG: IgG purified from serum samples of 10 healthy controls; RNP antibody: commercial snRNP monoclonal antibody; cont-Ab: commercial anti-rabbit IgG. Scale bar, 50 μm. (B) Incubation of GBS-IgG with snRNP antibodies or anti-snRNP antibody caused a significant increase in 10-kDa dextran permeability, compared with that with cont-IgG or cont-Ab, using a monolayer coculture model consisting of FH-BNB cells and <t>pericytes.</t> (C) GBS-IgG with anti-snRNP antibody induced a significant increase in the permeability of FH-BNB compared with cont-IgG, GBS-IgG without anti-snRNP antibody, or GBS-IgG after the reduction of U1-snRNP antibody from GBS-IgG with U1-snRNP antibody. (D) Incubation with GBS-IgG or snRNP antibodies leads to a decrease in claudin-5 and an increase in 10-kDa dextran permeability compared with cont-IgG using the spheroid model, which consists of both FH-BNB cells and pericytes (Left). Scale bar, 50 μm. Permeability of 20-kDa dextran increased after exposure to GBS-IgG or snRNP antibodies compared with cont-IgG using a microfluidic in vitro BNB coculture model (Right). Scale bar, 100 μm. BNB = blood-nerve barrier; U1-snRNP = U1-small nuclear ribonucleoprotein.
The Peripheral Nerve: Structure, Function And Reconstruction, supplied by Hampton Press, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peripheral+nerves/us07732200-24-4-31?v=Hampton+Press
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the peripheral nerve: structure, function and reconstruction - by Bioz Stars, 2026-07
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Image Search Results


(A) Immunostaining of human peripheral nerve microvascular endothelial cells (PnMECs) for snRNPs. Nuclear snRNP expression in monolayer PnMECs decreased after incubation with GBS-IgG with snRNP antibodies or anti-snRNP monoclonal antibody compared with after cont-IgG or cont-Ab. GBS-IgG: IgG purified from serum samples of 20 patients with U1-snRNP autoantibody–positive GBS; cont-IgG: IgG purified from serum samples of 10 healthy controls; RNP antibody: commercial snRNP monoclonal antibody; cont-Ab: commercial anti-rabbit IgG. Scale bar, 50 μm. (B) Incubation of GBS-IgG with snRNP antibodies or anti-snRNP antibody caused a significant increase in 10-kDa dextran permeability, compared with that with cont-IgG or cont-Ab, using a monolayer coculture model consisting of FH-BNB cells and pericytes. (C) GBS-IgG with anti-snRNP antibody induced a significant increase in the permeability of FH-BNB compared with cont-IgG, GBS-IgG without anti-snRNP antibody, or GBS-IgG after the reduction of U1-snRNP antibody from GBS-IgG with U1-snRNP antibody. (D) Incubation with GBS-IgG or snRNP antibodies leads to a decrease in claudin-5 and an increase in 10-kDa dextran permeability compared with cont-IgG using the spheroid model, which consists of both FH-BNB cells and pericytes (Left). Scale bar, 50 μm. Permeability of 20-kDa dextran increased after exposure to GBS-IgG or snRNP antibodies compared with cont-IgG using a microfluidic in vitro BNB coculture model (Right). Scale bar, 100 μm. BNB = blood-nerve barrier; U1-snRNP = U1-small nuclear ribonucleoprotein.

Journal: Neurology® Neuroimmunology & Neuroinflammation

Article Title: Small Nuclear Ribonucleoprotein Autoantibody Associated With Blood-Nerve Barrier Breakdown in Guillain-Barré Syndrome

doi: 10.1212/NXI.0000000000200405

Figure Lengend Snippet: (A) Immunostaining of human peripheral nerve microvascular endothelial cells (PnMECs) for snRNPs. Nuclear snRNP expression in monolayer PnMECs decreased after incubation with GBS-IgG with snRNP antibodies or anti-snRNP monoclonal antibody compared with after cont-IgG or cont-Ab. GBS-IgG: IgG purified from serum samples of 20 patients with U1-snRNP autoantibody–positive GBS; cont-IgG: IgG purified from serum samples of 10 healthy controls; RNP antibody: commercial snRNP monoclonal antibody; cont-Ab: commercial anti-rabbit IgG. Scale bar, 50 μm. (B) Incubation of GBS-IgG with snRNP antibodies or anti-snRNP antibody caused a significant increase in 10-kDa dextran permeability, compared with that with cont-IgG or cont-Ab, using a monolayer coculture model consisting of FH-BNB cells and pericytes. (C) GBS-IgG with anti-snRNP antibody induced a significant increase in the permeability of FH-BNB compared with cont-IgG, GBS-IgG without anti-snRNP antibody, or GBS-IgG after the reduction of U1-snRNP antibody from GBS-IgG with U1-snRNP antibody. (D) Incubation with GBS-IgG or snRNP antibodies leads to a decrease in claudin-5 and an increase in 10-kDa dextran permeability compared with cont-IgG using the spheroid model, which consists of both FH-BNB cells and pericytes (Left). Scale bar, 50 μm. Permeability of 20-kDa dextran increased after exposure to GBS-IgG or snRNP antibodies compared with cont-IgG using a microfluidic in vitro BNB coculture model (Right). Scale bar, 100 μm. BNB = blood-nerve barrier; U1-snRNP = U1-small nuclear ribonucleoprotein.

Article Snippet: After loading the cell suspension with human peripheral nerve pericyte (2.5 × 10 6 cells/mL) in collagen I gel (2 μL) into the middle channel, PnMECs were seeded and cultured in the top channel to establish a BNB microfluidic coculture model. CB medium and fresh Sciencell pericyte medium were used to assess their influence on barrier function.

Techniques: Immunostaining, Expressing, Incubation, Purification, Permeability, In Vitro